FIGURE 6.

BSO treatment enhances macrophage CD36 translational efficiency. A, RAW264.7 cells were treated with 5 μm cycloheximide (CHX) or cycloheximide plus 5 μm BSO for the indicated times followed by determination of CD36 protein expression. B, RAW264.7 cells were treated with 5 μm BSO for 16 h. The cells were then used to prepare polysomal RNA fractions followed by determination of CD36 and GAPDH mRNA expression by Northern blot analysis. C, RAW264.7 cells were treated with BSO at the indicated concentrations for 12 h followed by treatment with puromycin (10 μg/ml) for 1 h. After treatment, the cellular protein was extracted and used to conduct input assay by Western blot (IB) with anti-puromycin antibody (left panel). After input assay, the protein samples were used to conduct immunoprecipitation (IP) with anti-CD36 antibody or normal IgG followed by Western blot with anti-puromycin antibody for newly synthesized CD36 protein or with anti-CD36 antibody for whole CD36 protein expression (right panel).