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Comparison of ribonucleotide incorporation kinetics for polymerase families A, B, D, Y, and RT
| Polymerase | Family | kpol | Kd(rNTP) | kpol/Kd(rNTP) | Selectivity |
|---|---|---|---|---|---|
| s−1 | μm | μm−1s−1 | |||
| KF (45) | A | (4.7 ± 2.5) × 10−2 | 21 ± 7 | 2.3 × 10−3 | 3.4 × 103 |
| RB69 (24) | B | 0.74 ± 0.2 | (1.6 ± 0.4) × 10−4 | 4.6 × 10−5 | 6.4 × 104 |
| 9°N polD (this work) | D | 0.16 ± .01 | 360 ± 60 | 4.4 × 10−4 | 2.3 × 103 |
| Dbh (47) | Y | (1.4 ± 0.3) × 10−5 | 770 ± 360 | 1.8 × 10−8 | 3.4 × 103 |
| HIV-1 (49) | RT | 0.03 | 820 ± 150 | 3.7 × 10−5 | 1.3 × 105 |
