TABLE 5.
Comparison of pre-steady-state nucleotide incorporation kinetics for polymerase families A, B, C, D, X, Y, and RT
| Polymerase | kpol | Kd(dNTP) | kpol/Kd |
|---|---|---|---|
| s−1 | μm | μm−1 s−1 | |
| Family A | |||
| KF (21) | 50 | 5.5 | 9.0 |
| T7 (50) | 120 | 2 | 60 |
| Klentaq (51) | 21 | 35 | 0.60 |
| Family B | |||
| Vent polB (25) | 66 | 70 | 0.95 |
| RB69 (52) | 200 | 69 | 2.9 |
| T4 (53) | >400 | 20 | 20 |
| Human pol ϵ (27) | 248 | 31 | 8 |
| Yeast pol δ (54) | 0.93 | 24 | 0.04 |
| Family C | |||
| Sau PolC (55) | 180 | 4 | 45 |
| Family D | |||
| 9°N polD (this work) | 3.1 | 1.7 | 1.8 |
| Family X | |||
| rPolβ (23) | 12.5 | 1.9 | 6.6 |
| Family Y | |||
| Dpo4 (40) | 7.6 | 70 | 0.10 |
| Reverse transcriptase | |||
| HIV-1 (56) | 26 | 9 | 2.88 |