TABLE 1.

Primer and probe sequences

Primer name	Application	Sequence
pGexFOXD3 forward	Cloning	ATG GAT CCA TGA CCC TCT CCG GC
pGexFOXD3 reverse	Cloning	CTG AAT TCA TAT GAC CCT CTC CGG C
FOXDN forward	Cloning	CAG GAT CCA TGC GGC GCC GGA AAC GCT TCA A
FOXDN reverse	Cloning	CAG AAT TCC TAT TGC GCC GGC CAT TTG GCT T
FOX enhancer forward	Cloning	TCG AGT GTT TGC CCG CTA TTG TTA TTT TCC CCC GTT TGT TGT TTT CCC C
FOX enhancer reverse	Cloning	CAT GGG GGA AAA CAA CAA ACG GGG GAA AAT AAC AAT AGC GGG CAA ACA C
hGAPDH forward	RT-PCR	ACA TCA TCC CTG CCT CTA CT
hGAPDH reverse	RT-PCR	CTC TCT TCC TCT TGT GCT CTT G
CXCR4 canonical forward	RT-PCR	CCG AGG GCC TGA GTG CTC CAG
CXCR4 noncanical forward	RT-PCR	GCA GAG GAG TTA GCC AAG ATG
CXCR4 reverse	RT-PCR	ATC CAT TGC CCA CAA TGC CAG
CXCR4 round1 forward	ChIP	TTT GTT GGC TGC GGC AGC AGG
CXCR4 round1 reverse	ChIP	TTT TGG AGT ACG GGT ACC TCC
CXCR4 nested forward	ChIP	TAG CAA AGT GAC GCC GAG GG
CXCR4 nested reverse	ChIP	AAT GTC CTG GCC GCT TCT GC
β-Tubulin round1 forward	ChIP	AAA GGC CAC TAC ACA GAG GG
β-Tubulin round1 reverse	ChIP	TAC CAA CTG ATG GAC GGA GAG G
β-Tubulin nested forward	ChIP	TTG ATT CTG TCC TGG ATG TGG
β-Tubulin nested reverse	ChIP	TCA GAC ACT TTG GGT GAA GGC
CXCR4 WT forward	EMSA	CTT GAG TCC CGC CGC GCG CGG CGG CTT GCA CGC TGT TTG CAA A
CXCR4 WT reverse	EMSA	CTT ACG TTT GCA AAC AGC GTG CAA GCC GCC GCG CGC GGC GGG ACT
CXCR4 ΔP1 forward	EMSA	CTT GAT TAA TTA CGC GCG CGG CGG CTT GCA CGC TGT TTG CAA A
CXCR4 ΔP1 reverse	EMSA	CTT ACG TTT GCA AAC AGC GTG CAA GCC GCC GCG CGC GTA ATT AAT
CXCR4 ΔP2 forward	EMSA	CTT GAG TCC CGC CGC GCG CGG CGG CTT TAA TTA TGT TTG CAA A
CXCR4 ΔP2 reverse	EMSA	CTT ACG TTT GCA AAC ATA ATT AAA GCC GCC GCG CGC GGC GGG ACT
CXCR4 ΔF forward	EMSA	CTT GAG TCC CGC CGC GCG CGG CGG CTT GCA CGC GGC CGG CAA A
CXCR4 ΔF reverse	EMSA	CTT ACG TTT GCC GGC CGC GTG CAA GCC GCC GCG CGC GGC GGG ACT
CXCR4 ΔP1ΔP2 forward	EMSA	CTT GAA CTC AGC CGC GCG CGG CGG CTT GGG CGA TGT TTG CAA A
CXCR4 ΔP1ΔP2 reverse	EMSA	ACG TTT GCA AAC ATC GCC CAA GCC GCC GCG CGC GGC TGA GTT