TABLE 1.
Primers used for RT-PCR
Re, reverse; Fw, forward; odc, ornithine decarboxylase.
| Gene | Sequence (5′–3′) | Ref. |
|---|---|---|
| ets1a Fw | TTGAACAGGCAATAGCAG | This study |
| ets1a Re | ACATCTGTAGTCCCTCCC | |
| id3 Fw | GGGAAACTTTGAGAGAGAGAGAG | This study |
| id3 Re | CGGGTTGCAAAGGGTTAAAG | |
| snail2 Fw | TCCCGCACTGAAAATGCCACGATC | 22 |
| snail2 Re | CCGTCCTAAAGATGAAGGGTATCCTG | |
| sox9 Fw | AAGCAGAATGTCCTCTGTGA | 35 |
| sox9 Re | AAGGCCAGATTCAGTTCTTC | |
| foxd3 Fw | GGAGGGAGGGGGCAATGCAC | 12 |
| foxd3 Re | CCCCGAGCTCGCCTACT | |
| odc Fw | CAGCTAGCTGTGGTGTGG | E. M. De Robertis |
| odc Re | CAACATGGAAACTCACACC | |
| pax3 Fw | CTACCTCGGTTTCTTGACTG | 35 |
| pax3 Re | TGGTCAATCCTTCTTAATGG | |
| zic1 Fw | ATGAAGGTCCACGAAGCATC | 8 |
| zic1 Re | CGTGCTGTGATTGGACGTGT | |
| sox2 Fw | GAGGATGGACACTTATGCCCAC | E. M. De Robertis |
| sox2 Re | GGACATGCTGTAGGTAGGCGA | |
| sox3 Fw | TGATGCAGGACCAGTTGGGC | 35 |
| sox3 Re | TGAAGTGAAGGGTCGCTGGC | |
| msx1 Fw | GCTAAAAATGGCTGCTAA | E. M. De Robertis |
| msx1 Re | AGGTGGGCTGTGTAAAGT |