Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 28;290(36):22019–22029. doi: 10.1074/jbc.M115.674127

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Knockdown of Bcor promotes syncytiotrophoblast differentiation of TSCs. A–C, Bcor knockdown TSCs (shBcor-8 and shBcor-9) and control TSCs (shControl) were cultured in TS medium (A), TS+Ac10 medium (B), or TS medium plus 10 μm SB431542 (TS+SB10 medium) (C) to allow differentiation for 0, 2, 4, and 6 days before being harvested for qRT-PCR analysis of the syncytiotrophoblast markers Gcm1, Synb, and Cebpa. D and E, cells differentiated in TS medium for 4 days were harvested for anti-GCM1 immunostaining (D) and immunoblotting (E). Scale bar: 10 μm. Five fields of each sample were quantified for the ratio of GCM1-positive cells. Data are presented as mean ± S.E. (n = 3; ***, p < 0.001; **, p < 0.01; *, p < 0.05).