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. 2015 Jul 16;290(36):22061–22075. doi: 10.1074/jbc.M115.649657

FIGURE 7.

FIGURE 7.

Mutant CPCs have impaired mitochondrial respiration and reduced membrane potential. A, mitochondrial respiration was measured in WT and POLG CPCs using a Seahorse XF analyzer. Maximal oxygen consumption rate (OCR) was normalized to cell number (n = 4). Cell seeding density: 10K = 10,000; 20K = 20,000. FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone. B, representative fluorescent images and quantitation of tetramethylrhodamine methyl ester (TMRM) fluorescence in WT and POLG CPCs (n = 3). Scale bar = 25 μm. Units are arbitrary. C, analysis of cellular ATP content in WT and POLG CPCs (n = 3). D, l-lactate present in growth medium was measured in WT and POLG CPCs (n = 3). E, 2-deoxyglucose (10 nm) was added to the growth medium to inhibit glycolysis in WT and POLG CPCs. Cell death was assessed using YO-PRO-1 staining (n = 3). **, p < 0.01; ***, p < 0.001 versus WT; n.s., not significant.