FIGURE 8.

Activation of differentiation leads to down-regulation of cytosolic glycolytic enzymes in both WT and POLG CPCs. A, WT and POLG CPCs were incubated in differentiation medium, and cell death was assessed using YO-PRO-1 staining 4 days later (n = 3). B, representative Western blots of glycolytic enzymes after incubation in DM. C, quantitation of hexokinase I, hexokinase II, PKM1/2, and GAPDH at different time points after differentiation (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001.