FIGURE 2.

DNase I footprints of ETS1Δ280-DNA complexes. Radiolabeled DNA bearing MMP3 wild type (A), MMP3 M1 (B), or p53 (C) were incubated with increasing amounts of ETS1Δ280. Complexes were then subjected to DNase I cleavage, and the resulting DNA fragments were resolved via denaturing PAGE. Shown is a PhosphorImager scan of the gel. In each panel, the lane labeled with a − contains uncleaved DNA, and the lane labeled with 0 contains DNA incubated with DNase I in the absence of protein. Positions of bases protected from cleavage by ETS1Δ280 binding are bracketed. Enhancements of cleavage are noted with asterisks.