FIGURE 3.

ETS1Δ280 R378C dimer formation. A, shown is an immunoblot of ETS1Δ280 R378C cross-linked by BMOE in the absence or presence of the indicated DNA sequence. dI-dC refers to poly(dI-dC), a repeating inosine-cytosine oligonucleotide used as a nonspecific binding template. The positions of protein monomers and dimers are indicated. Intermediate bands between monomers and dimers are thought to result from incomplete synthesis of ETS1 protein when purified from E. coli. B, quantification of results seen in A. The error bars represent standard deviation derived from four or more replicate experiments.