FIGURE 4.

Analysis of ETS1Δ280 conformational states. A, partial proteolysis of ETS1Δ280 in the absence or presence various DNA sequences. Shown is a Coomassie-stained SDS-PAGE of the products of a limited, time-dependent proteolytic cleavage of ETS1Δ280 by chymotrypsin in the absence or presence of the indicated DNA. The electrophoretic mobilities of molecular weight standards are indicated to the left of the gel. B, circular dichroism spectra of ETS1 in the absence or presence of DNA. Similar to A, ETS1Δ280 was saturated with either specific (MMP3 wild type) or nonspecific (MMP3 M1) DNA. The reaction mixture CD spectra was then measured and converted to molar ellipticity (Mol. elipt.). The spectra for ETS1 alone is represented with a solid line, ETS1 in the presence of MMP3 WT is represented with a dotted line, and ETS1 in the presence of MMP3 M1 is represented with a dashed line.