TABLE 1.

Affinity of ETS1Δ280 for wild-type and mutant ETS1 binding sites

The sequences and affinity (dissociation constants, K_D) ± standard deviation of ETS1Δ280 for naturally occurring and mutant binding sites were determined by EMSA (see “Experimental Procedures”). EBSs present in each sequence are underlined. NS indicates “nonspecific,” where ETS1Δ280 formed no identifiable complex with the DNA site.

DNA	Core sequence	KD
		nm
MMP3 WT	ACAGGAAGCACTTCCTGG	0.20 ± 0.058
MMP3 M1	ACAAAAAGCACTTCCTGG	NS
MMP3 M2	ACAGGAAGCACTTTTTGG	NS
MMP3 + 4	AGGAAGCACGCACTTCCT	NS
p53	TTAGGAAGGCTTTCCGTA	6.2 ± 3.6