FIGURE 2.

FGF9 suppresses metastasis of Renca cell-derived renal tumors. A, photographs of kidney tumors harvested 14 days after renal subcapsular injection of Renca cells expressing either GFP or FGF9 in growth factor-reduced Matrigel. The corresponding, non-injected contralateral kidney is shown on the right of each photograph. Western blot showing detectable FGF9 expression Renca-FGF9 tumor is shown on the right. B, graph of kidney weights. *, p = 0.010 for GFP-expressing tumor versus right kidney; *, p = 0.003 for FGF9-expressing tumor versus right kidney; p = 0.89 for GFP- versus FGF9-expressing tumor (n = 6). C, graphs depicting the proliferation (left) and apoptosis (right) in renal tumors, as assessed by immunostaining for Ki-67 and TUNEL assay, respectively. D, micrographs of lungs harvested from mice 14 days after renal injection of Renca cells expressing GFP or FGF9. Macrometastases are evident as translucent distensions on the lung surface, with selected lesions in a digitally magnified region (box) depicted by the arrows. E, photomicrographs of 5-μm formalin-fixed lung sections stained with hematoxylin and eosin showing metastatic foci within lung parenchyma. F, graphs depicting the mean number of surface macro-metastases (*, p = 0.029), the density of intrapulmonary metastases (*, p = 0.004), and the size of metastatic foci (*, p = 0.019) in control and FGF9-expressing tumors.