TABLE 1.
Primer sequences used for polymerase chain reactions
| Gene | Forward primer | Reverse primer |
|---|---|---|
| Fgfr1 | ATCCTCGGAAGATGATGACG | GAAGGCACCACAGAATCCAT |
| Fgfr2 IIIb | AGGAGCACCGTACTGGACCAACA | GCAGACTGGTTGGCCTGCCC |
| Fgfr2 IIIc | GGATGGGGTGCACTTGGGGC | CGGCGGCCTTCAGGACCTTG |
| Fgfr3 IIIb | ATTTCATAGGCGTGGCTGAG | GACAAGGGGTGTGTTGGAGT |
| Fgfr3 IIIc | CCACCGACAAGGAGCTAGAG | TCGCATCATCTTTCAGCATC |
| Fgfr4 | CTTCCTTCCTGAGGATGCTG | CTGTCCATCCTTGAGCCAGT |
| Actb | TGGGTCAGAAGGATTCCTATGT | TGTACGTTGCTATCCAGGCTG |
| Pdgfb | AATGCTGAGCGACCACTCCATC | TCGGGTCATGTTCAAGTCCAGC |
| Pdgfd | CTGTCAACTGGAAGTCCTGCAC | CCCTTCTCTTGAAATGTCCAGGC |
| Pdgfra | CTGGCTCGAAGTCAGATCCACA | GACTTGTCTCCAAGGCATCCTC |
| Pdgfrb | GTGGTCCTTACCGTCATCTCTC | GTGGAGTCGTAAGGCAACTGCA |
| Dll1 | GCTGGAAGTAGATGAGTGTGCTC | CACAGACCTTGCCATAGAAGCC |
| Dll4 | GGGTCCAGTTATGCCTGCGAAT | TTCGGCTTGGACCTCTGTTCAG |
| Jag1 | TGCGTGGTCAATGGAGACTCCT | TCGCACCGATACCAGTTGTCTC |
| Jag2 | CGCTGCTATGACCTGGTCAATG | TGTAGGCGTCACACTGGAACTC |
| Notch1 | GCTGCCTCTTTGATGGCTTCGA | CACATTCGGCACTGTTACAGCC |
| Notch2 | CCACCTGCAATGACTTCATCGG | TCGATGCAGGTGCCTCCATTCT |
| Notch3 | GGTAGTCACTGTGAACACGAGG | CAACTGTCACCAGCATAGCCAG |
| Notch4 | GGAGATGTGGATGAGTGTCTGG | TGGCTCTGACAGAGGTCCATCT |
| Angpt1 | AACCGAGCCTACTCACAGTACG | GCATCCTTCGTGCTGAAATCGG |
| Angpt2 | GCATGACCTAATGGAGACCGTC | GATAGCAACCGAGCTCTTGGAG |
| Shh | ACCCAACTCCGATGTGTTCCGTTA | TATATAACCTTGCCTGCCGCTGCT |
| Ihh | CGGCTTCGACTGGGTGTATTAC | AGGAAAGCAGCCACCTGTCTTG |
| Sphk1 | GCTTCTGTGAACCACTATGCTGG | ACTGAGCACAGAATAGAGCCGC |
| S1pr1 | CGCAGTTCTGAGAAGTCTCTGG | GGATGTCACAGGTCTTCGCCTT |
| Apln | AAAGCTTGCACAAATAGGCCCTGG | TTCCTAGCCACCCGCTTCACTAAA |
| Aplnr | GCCATTTCCGAAAGGAGCGCAT | CTTCACCAGGTGGTAAGGCATC |