FIGURE 1.

LPA initiates epithelial-to-mesenchymal transition in epithelial ovarian carcinoma cells. Multicellular aggregates formed from OVCA429 or OVCA433 cells were untreated or treated with 40 μm LPA overnight and stained for expression and localization of E-cadherin and vimentin (anti-E-cadherin, green, 1:300; anti-vimentin, red, 1:200) (A–D) or vinculin and F-actin (anti-vinculin, green, 1:100; rhodamine phalloidin labeling, red) (E–H). Nuclei were stained with DAPI (blue). Magnification ×20.