FIGURE 5.
Examples of progress curves for HjCBM hydrolyzing Avicel at different temperatures. The points are raw data from biosensor measurements, and the lines are best fits of a kinetic model describing processive cellulases (see “Results” for details). The initial, sigmoidal part of the curves (e.g. the first ∼30 s at 25 °C) is the so-called burst phase, and the subsequent, near-linear course reflects quasi-steady state. The enzyme concentration was 150 nm, and the Avicel load was 3.0 g/liter at 5 °C and 5.0 g/liter at 25 and 50 °C.