FIGURE 1.
IL1R2 expression is higher in human CRC cells than normal colon epithelial cells and correlates well with IL-6 mRNA levels in tumor tissues of CRC patient. A, the IL1R2 protein levels in normal colon cells (FHC) and eight human CRC cell lines were measured by Western blot analysis. β-Actin served as a loading control. B, the nuclear and cytosolic IL1R2 in FHC and CRC cells was examined by Western blotting. GAPDH served as the loading control for the cytosolic fraction and histone H3 for the nuclear fraction. The Western blots (A and B) were independently repeated at least three times, and representative data are shown. C, correlation between the expression of IL1R2 and IL-6 mRNA (ΔΔCt indicates ΔCt of target gene − ΔCt of GAPDH) in CRC patients. The correlation between IL1R2 and IL-6 was analyzed using the χ2 test.