Skip to main content
. 2015 Jul 24;290(36):22212–22224. doi: 10.1074/jbc.M115.644823

FIGURE 6.

FIGURE 6.

CM harvested from IL1R2-overexpressing cells is enriched with IL-6 and VEGF. A, ELISA was used to measure the IL-6 and VEGF-A levels in the serum-free CM harvested from CRC cells with modulated IL1R2 expression. The protein levels are means ± S.D. of three independent experiments. *, p < 0.05 by Student's t test. B and C, the proliferation (B) and migration (C) of EA.hy926 cells were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (48 h, 10% FCS-containing CM) and Transwell migration assays (8 h, serum-free CM in the upper chamber and 5% FCS-containing medium in the lower chamber), respectively, after incubation in CM (with or without anti-IL-6 (2 μg/ml), anti-VEGF-A (2 μg/ml), or rabbit IgG control (2 μg/ml)) harvested from HT29-shV, HT29-shIL1R2, HCT116-Vec, and HCT116-IL1R2 cells. The data of relative proliferation and migration ability are means ± S.D. of three independent experiments. *, p < 0.05 by Student's t test.