The roles of IL1R2, IL-6, and VEGF-A in A0, A2, and T4R2 cells.
A, the protein levels of IL1R2, IL-6, and VEGF-A in A0, A1, A2, and T4R2 cells were analyzed by Western blotting. β-Actin served as a loading control. B, the protein levels of IL1R2, IL-6, and VEGF-A in T4R2-shV, T4R2-shIL1R2, HaCaT-Vec, HaCaT-IL1R2-1, and HaCaT-IL1R2-2 cells were analyzed by Western blotting. C, DIVAA assay was performed as described for Fig. 7B. The CM was harvested from T4R2-shV, T4R2-shIL1R2, HaCaT-Vec, HaCaT-IL1R2-1, and HaCaT-IL1R2-2 cells. D and E, a luciferase assay was used to analyze the activity of wild-type or mutant IL-6 and VEGF-A promoter in T4R2-shV and T4R2-shIL1R2 cells (D) and HaCaT-Vec, HaCaT-IL1R2-1 and HaCaT-IL1R2-2 cells (E). The Western blots were independently repeated at least three times, and the representative data are shown. The relative invasion and promoter activity are means ± S.D. of three independent experiments. *, p < 0.05 by Student's t test.