FIGURE 3.

Disruption of CAP-ligand interactions through the use of cAMP analogs. a, the distribution of binding interactions between cAMP and wild type CAP. b–d, binding interactions between Sp-cAMPS (b), cIMP (c), and 2′-deoxy-cAMP (d) and CAP. e–g, ITC trace (upper panel) and binding isotherm (lower panel, the different colored symbols represent individual experiments) for the calorimetric titration of cAMP (e), Sp-cAMPS (f), and cIMP (g) to CAP are shown. The thermodynamic parameters obtained are shown in Table 2.