TABLE 3.
Binding parameters for the first and second ligand binding events
The mean values ± S.E. are given for wild type CAP for the first and second ligand binding events. n is provided in parentheses. All units are kcal mol−1.
| Ligand | ΔH1 | ΔH2 | ΔG1 | ΔG2 | −TΔS1 | −TΔS2 |
|---|---|---|---|---|---|---|
| cAMP | −2.2 ± 0.1 (5) | 7.6 ± 0.1 (5) | −7.3 ± 0.1 (5) | −7.0 ± 0.1 (5) | −5.1 ± 0.1 (5) | −14.7 ± 0.2 (5) |
| Sp-cAMPS ENMR82/S83 | −3.5 ± 0.3 (6) | 0.9 ± 0.6 (6) | −6.2 ± 0.1 (6) | −5.1 ± 0.2 (6) | −2.7 ± 0.4 (6) | −5.9 ± 0.5 (6) |
| cIMP ENMT127/S128 | −3.8 ± 0.1 (4) | −2.2 ± 0.1 (4) | −6.9 ± 0.0 (4) | −5.9 ± 0.1 (4) | −3.2 ± 0.1 (4) | −3.7 ± 0.1 (4) |