FIGURE 2.

Effect of knockdown of each human Rab isoform on the morphology of the Golgi in HeLa-S3 cells. A, screening for Rabs whose knockdown induced fragmentation of the Golgi. Rabs whose knockdown resulted in Golgi fragmentation in more than 40% of the siRNA-transfected cells were considered candidates for Rabs involved in the morphology of the Golgi (blue bars). B, typical images of Rab knockdown HeLa-S3 cells. HeLa-S3 cells were transfected with each Rab siRNA or control siRNA. Three days after transfection, the cells were fixed and stained with anti-GM130 antibody (a Golgi marker; green) and DAPI (a nucleus marker; blue). The asterisks indicate the cells that exhibited Golgi fragmentation. High magnification views of the boxed areas are shown in the lower panels, and the cells are outlined with a broken line. Scale bars, 20 μm.