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. 2015 Jul 24;290(36):22250–22261. doi: 10.1074/jbc.M115.669242

FIGURE 5.

FIGURE 5.

Non-redundant roles of Rab1A, Rab1B, Rab2A, Rab2B, and Rab6B in the compacted Golgi morphology. HeLa-S3 cells were transfected with Rab1A siRNA (A), Rab1B siRNA (B), Rab2A siRNA (C), Rab2B siRNA (D), Rab6B siRNA (E), and Rab8A siRNA (F) together with pEGFP-C1 or pEGFP-C1-Rab1A/1B/2A/2B/6B/8A/8B/29 (or their RabSR mutant). Three days after transfection, the cells were fixed and stained with anti-GM130 antibody and DAPI. EGFP-expressing cells and EGFP-Rab-expressing cells were identified by green fluorescence, and their Golgi morphology was analyzed. The bars represent the means and S.D. (error bars) of data from three independent experiments. *, p < 0.05; **, p < 0.01, Dunnett's test. Note that the Golgi fragmentation phenotype induced by knockdown of Rab1A, Rab1B, Rab2A, Rab2B, and Rab6B was most effectively rescued by re-expression of Rab1ASR, Rab1BSR, Rab2ASR, Rab2BSR, and Rab6BSR, respectively (blue bars). Approximately 30–40% of the Rab1A/1B/2A/2B/6BSR-expressing cells (blue bars) still exhibited the Golgi fragmentation phenotype presumably because of the low co-transfection efficiency of the siRNAs and plasmids.