TABLE 1.
Subset of proteins with greater or lower abundance in untreatred kin− S49 relative to untreated WT (kin−:WT ratio >1.5 or <0.67)
kin− cells a displayed higher and lower abundance of proteins within DAVID categories related to metabolism and oxidation/reduction. A full listing of basally differential proteins is contained in supplemental Table 2. Data for proteins whose expression increased are shown in bold; proteins with decreased expression are shown in italics.
| Gene symbol | Accession no. | Average ratio | Protein | Unique peptide no. | Spectrum no. | % Protein coverage |
|---|---|---|---|---|---|---|
| Metabolic process | ||||||
| Pck2 | IPI00223060 | 4.87 | Pck2 mitochondrial phosphoenolpyruvate carboxykinase 2 | 25 | 139 | 38 |
| Tars2 | IPI00132419 | 3.91 | Tars2 threonyl-tRNA synthetase, mitochondrial precursor | 12 | 40 | 19 |
| Gm2296 | IPI00851029 | 3.75 | Ubiquitin-conjugating enzyme E2S pseudogene | 6 | 12 | 28 |
| Aup1 | IPI00137725 | 3.25 | Aup1 ancient ubiquitous protein | 6 | 39 | 19 |
| Gart | IPI00230612 | 3.03 | Gart phosphoribosylglycinamide formyltransferase | 12 | 30 | 17 |
| Gls | IPI00671957 | 2.57 | Gls glutaminase isoform 5 | 22 | 242 | 3 |
| Usp39 | IPI00457815 | 2.23 | Usp39 U4/U6.U5 tri-snRNP-associated protein 2 | 7 | 9 | 13 |
| Nfs1 | IPI00311072 | 1.97 | Nfs1 Adult male kidney cDNA, RIKEN full-length enriched library, clone:0610009K17 product:nitrogen fixation gene 1 | 12 | 51 | 31 |
| LOC100047372 | IPI00222546 | 1.91 | Rpl22,LOC100047372 60S ribosomal protein L22 | 8 | 117 | 47 |
| Hk2 | IPI00114342 | 1.59 | Hk2 hexokinase-2 | 55 | 1122 | 44 |
| Gfm1 | IPI00230283 | 1.51 | Gfm1 elongation factor G 1, mitochondrial precursor | 20 | 119 | 27 |
| MPC2 | IPI00131896 | 0.64 | Mitochondrial pyruvate carrier 2 | 7 | 23 | 29 |
| Tmlhe | IPI00129163 | 0.60 | Tmlhe trimethyllysine dioxygenase, mitochondrial precursor | 5 | 13 | 8 |
| Taok3 | IPI00670075 | 0.44 | Taok3 similar to Serine/threonine-protein kinase TAO3 (thousand and one amino acid protein 3) isoform 9 | 5 | 9 | 6 |
| Ssb | IPI00134300 | 0.36 | Ssb lupus La protein homolog | 9 | 18 | 19 |
| Lipid metabolic process | ||||||
| Osbp | IPI00755161 | 1.61 | Osbp oxysterol binding protein | 6 | 8 | 9 |
| Cyb5r3 | IPI00759904 | 1.53 | Cyb5r3 Isoform 2 of NADH-cytochrome b5 reductase 3 | 12 | 56 | 34 |
| Gpam | IPI00387288 | 0.57 | Gpam glycerol-3-phosphate acyltransferase, mitochondrial | 8 | 46 | 12 |
| Fech | IPI00608064 | 0.25 | Fech Fech protein (fragment) | 10 | 36 | 9 |
| Oxidation/reduction | ||||||
| Mthfd2 | IPI00109824 | 3.36 | Mthfd2 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial precursor | 20 | 223 | 69 |
| LOC100046934 | IPI00854030 | 2.98 | LOC100046934 similar to amine oxidase (flavin-containing) domain 2 | 8 | 20 | 11 |
| Ndufv3 | IPI00128285 | 0.67 | NADH dehydrogenase (ubiquinone) flavoprotein 3 | 9 | 19 | 22 |
| Ndufa12 | IPI00344004 | 0.54 | Ndufa12 NADH dehydrogenase (ubiquinone) 1α subcomplex, 12 | 7 | 27 | 54 |