TABLE 3.

Morphologic characteristics of mitochondria of WT and kin⁻S49 cells

Measurements derived from EM micrographs of WT S49 cells (untreated or incubated with CPT-cAMP for 16 h) and kin⁻ S49 cells. Quantification of mitochondrial volume density (expressed as %, n =10), mitochondria number per μm² cytoplasmic area (n =10), and mitochondria size (n =20) is represented by cross-sectional area (μm²; n =10), and cristae abundance is represented by the ratio of total cristae membrane surface area to mitochondrial outer membrane surface area (n =10). CPT-cAMP-treated WT S49 cells had a lower volume density of mitochondria and smaller mitochondria but no change in mitochondria number compared to untreated WT cells. CPT-cAMP-treated WT cells also have mitochondria with fewer cristae. p values in boldface have a value of < 0.01.

	Mean	S.E.	t test vs. control	t test vs. kin−
Mitochondrial volume density (%)
WT untreated	14	0.79
WT CPT-cAMP	10	0.8	p = 0.0022	p = 0.13
kin−	13	1.3	p = 0.29
kin− CPT-cAMP	17	2.9		p = 0.15
Mitochondrial number/cytoplasmic area (μm2)a
WT untreated	0.14	0.017
WT CPT-cAMP	0.12	0.038
kin−	0.14	0.026
kin− CPT-cAMP	0.12	0.011
Mitochonrial cross-sectional area (μm2)
WT untreated	0.54	0.048
WT CPT-cAMP	0.35	0.075	p = 0.0015	p = 0.0032
kin−	0.61	0.045	p = 0.61
kin− CPT-cAMP	0.70	0.087		p = 0.37
Total cristae membrane SA/mito outer membrane SA
WT untreated	1.9	0.17
WT CPT-cAMP	0.48	0.11	p = 0.0000036	p = 0.000010
kin−	1.6	0.14	p = 0.17

^a Error was not significant in any comparison.