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. 2015 Jul 28;290(36):22298–22308. doi: 10.1074/jbc.M115.643361

FIGURE 6.

FIGURE 6.

CD300H ligation in CD16+ Mo induces cytokine and chemokine secretion. CD16+ Mo (A–E) and CD16− Mo (F and G) were isolated from peripheral blood mononuclear cells by using the MACS system. Their purity was then analyzed by using flow cytometry (A and F). MACS-isolated Mo from G/G (CD300H+) or A/A (CD300H−) genotype donors were stimulated with plate-coated F(ab′)2 fragment of TX93 or control Ig for 24 h (B, D, and G) or 4 h (C and E). Culture supernatant was analyzed by ELISA (B) or subjected to neutrophil migration assay (D) or cytometric bead array (G). Cells were analyzed for IL-6 (C) and chemokine (E) expression by using quantitative polymerase chain reaction. In D, culture supernatant was transferred to the lower compartment of transwells, and neutrophils were placed in the upper compartment. This was followed by a 30-min incubation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant. All data are representative of three independent experiments. Data in C and E are shown as mean ± S.D. (error bars) of four (CD300H+) and three (CD300H−) different donors.