FIGURE 7.

CD300H ligation in CD11c⁺ DCs induces IL-8 production. CD11c⁺ DCs were isolated from peripheral blood mononuclear cells by using the MACS system. A, the purity was analyzed by using flow cytometry. MACS-isolated DCs from G/G (CD300H⁺) or A/A (CD300H⁻) genotype donors were stimulated with plate-coated F(ab′)₂ fragment of TX93 or control Ig for 24 h. B, cells were analyzed for expression of co-stimulatory molecules by flow cytometry. C, culture supernatant was analyzed by cytometric bead array. Lineage: CD3, CD19, CD56, CD14. **, p < 0.01; NS, not significant. Data are representative of three independent experiments. Error bars, S.D.