Fig. 3.
NGF induction in human astrocytoma cell line U87 by PRP (a) and NP (b). U87 cells (1 × 106/ml) were incubated with TNFα (50 pg/ml) used as reference sample, PRP (0.1 and 10 μg/ml), or NP (0.1 and 10 μg/ml) for 24 h. Supernatants were harvested, concentrated, and then analyzed for NGF protein level by ELISA as described in Materials and Methods. Results are presented as median ± quartile (25–75 %) and min–max (n = 5–6). *p ≤ 0.05 statistically significant difference in sample value versus control