Fig. 2.

CCL21 mRNA expression in the CNS during ChREAE. The analysis of expression was performed using qPCR technique in the whole brain homogenates isolated during subsequent phases of ChREAE. 1 Naïve—nonimmunized control animals. 2–4 Groups of animals with ChREAE: first attack of ChREAE, remission and with the second attack. Expression was analyzed by qPCR as described in “Materials and methods”. n = 5–8 animals per group. Two identical experiments were done and results from the representative experiment are shown. Significance is denoted as *p < 0.05; **p ≤ 0.01