Table 2.
Summary of diversity described in European ecotypes by AMOVA
| Source | df | SS | MS | Est. var. | % |
|---|---|---|---|---|---|
| Between regions | 3 | 48,323 | 16,108 | 85 | 8 |
| Between accessions | 86 | 230,273 | 2678 | 247 | 24 |
| Between individuals | 626 | 446,237 | 713 | 713 | 68 |
| Total | 715 | 724,833 | 1045 | 100 |
| Stat. | Value | P(rand ≥ data) |
|---|---|---|
| PhiRT | 0.081 | 0.001 |
| PhiPR | 0.257 | 0.001 |
| PhiPT | 0.318 | 0.001 |
| Region | No. of individuals | No. of accessions | Between accessions (%) | Within accessions (%) | PhiPT | P value |
|---|---|---|---|---|---|---|
| “East” | 96 | 12 | 27 | 73 | 0.268 | 0.001 |
| “North” | 360 | 45 | 24 | 76 | 0.236 | 0.001 |
| “South” | 124 | 16 | 31 | 69 | 0.310 | 0.001 |
| “West” | 136 | 17 | 26 | 74 | 0.260 | 0.001 |
Df degrees of freedom; SS sum of squares, MS mean square, Est var. estimated variance. PhiRT = AR/(WP + AP + AR) = AR/TOT among regions. (among individuals within accessions). (among accessions within region) where AR is between regions; AP is between accessions within region; WP is between individuals within accession
Individuals divided into four regions as described by PCA (Fig. 1 and defined in Supplementary Fig. 1). Regions described as “North”—upper quadrant of PCA plot including individuals from the UK, Northern Europe and Spanish outliers. “West”—bottom left group comprised of individuals from Spanish and Portuguese sample sites. “South”—bottom quadrant comprising Italian, Bulgarian and Turkish individuals. “East” group describes individuals on centre right of PCA plot, including Romania, Czech Republic, Bulgaria