Table 1.
Methods used in six mass-spectrometry based proteomic studies
| Study Author, year | Subjects (n) AAAs/Controls | Sample | Depletion kit | Protein separation | Protein identification |
|---|---|---|---|---|---|
| Nordon, 2010 | 20/20 | Serum | IgY-12 | 2D-PAGE | LC-MS/MS |
| Gamberi, 2011 | 8/6 | Plasma | Not used | 2D-PAGE | MS/MS |
| Pulinx, 2011 | 24/0 | Serum | Not used | 2D-DIGE | MS/MS |
| Acosta-Martin, 2011 | 17 (pooled)/17 (pooled) | Plasma | MARS-14 | TMT | LC-MS/MS |
| Wallinder, 2012 | 4/4 | Plasma | ProteoMiner | 2D-DIGE | LC-MS/MS |
| Spadaccio, 2012 | 20/20 | Serum | PROT-BA | 2D-PAGE | MS |
Abbreviations: 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; 2D-DIGE, two-dimensional differential in-gel electrophoresis. LC, liquid-chromatography; MS, mass-spectrometry; MS/MS, tandem mass-spectrometry; TMT, tandem mass tags.