Table 3.
Number | Nucleotide sequence | Remarks | |||||
TTGG | GCCCGACGTC | GCATGCTCCC | GGCCGCCATG | GCGGCCGCGG | GAATTCGATT | Plasmid DNA | |
409-468 | TTCGATCCCA | AGAAGGAATC | AA1CCTTTGAA | GAGAGAAGTT | ACTGGCAATC | TCAAGTCAAC | HPA cDNA |
469-528 | CAGGATATTT | GCAAATATGG | ATCCATCCCT | CCTGATGTGG | AGGAA3,5AAGTT | ACGGTTGGAA | HPA cDNA |
529-588 | TGGCCCTACC | AGGAGCAATT | GCTACTCCGA | GAACACTACC | AGAAAAAGTT | CAAGAACAGC | HPA cDNA |
589-648 | ACCTACTCAA | GAAGCTCTGT | AGATGTGCTA | TACACTTTTG | CAAACTGCTC | AGGACTGGAC | HPA cDNA |
649-708 | TTGATCTTTG | GCCTAAATGC | GTTATTAAGA | ACAGCAGATT | TGCAGTGGAA | CAGTTCTAAT | HPA cDNA |
709-768 | GCTCAGTTGC | TCCTGGACTA | CTGCTCTTCC | AAGGGGTATA | ACATTTCTTG | GGAACTAGGC | HPA cDNA |
769-828 | AATGAACCTA | ACAGTTTCCT | TAAGAAGGCT | GATATTTTCA | TCAATGGGTC | GCAGTTAGGA | HPA cDNA |
829-888 | GAAGATTTTA | TTCAATTGCA | TAAACTTCTA | AGAAAGTCCA | CCTTCAAAAG4 | T6GCAAAACTC | HPA cDNA |
889-948 | TATGGTCCTG | ATGTTGGTCA | GCCTCGAAGA | AAGACGGCTA | AGATGCTGAA | GAGCTTCCTG | HPA cDNA |
949-993 | AAGGCTGGTG | GAGAAGTGAT | TGATTCAGTT | ACATGGCATC | ACTAC2 | HPA cDNA | |
AATCACTAGT | GAATTCGCGG | CCGCCTGCAG | GTCGACCATA | TGGGAGAGCT | CCCAACGCGT | Plasmid DNA | |
TGAATCACTA | GTGAATTCGC | GGCCGCCTGC | AGGTCGACCA | TATGGGAGAG | CTCCCAACGC | Plasmid DNA | |
GTTG | Plasmid DNA |
1,2The segments with italics and boldface corresponded to the primers used in RT-PCR. 3,4The letters represented the mutated bases. 5,6The parts with underline represented the codons.