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Expression of TLR4 and MD-2 mRNA by RT-PCR analysis. A: PCR products were electrophoresed on agarose gels and photographed. B: Quantitative data of mRNA levels were shown as the ratio of relative absorbance and expressed as mean ± SD. Expression of TLR4 and MD-2 mRNA were sig-nificantly increased in the IR group compared with the con-trol group (P < 0.01).
