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RT-PCR analysis of mRNA expression of Na+- HCO3- cotransporter (NBC) in colonic cells. A: RT-PCR results with products as expected of NBC found in rat colon (1), T84 (2) and Caco-2 (3) with positive control (4) using pancreatic duct cells (CAPAN-1) and negative control (5) where cDNA was omitted. B: Semi-quantitative analysis of NBC expression in Caco-2 (1) ; C: T84 (2) cells with NBC to GAPDH (internal marker) ratio shown.
