Fig. 2.

Effect of butyrate on human NHE8 gene promoter activity. Cells were cotransfected with pRL-CMV and pGL3 basic (pGL3b) or human NHE8 promoter constructs (pGL3b/-32, pGL3b/-1905). Butyrate was applied 16 h before measuring promoter activities. Promoter activity is calculated as a relative activity, a ratio of firefly luciferase activity driven by NHE8 promoter over Renilla luciferase activity driven by CMV promoter. The degree of stimulation on NHE8 promoter activity is shown as the ratio of relative activity in butyrate-treated cells over relative activity in control cells. Results are means ± SE from 3 separate experiments. *P < 0.05 for CT vs. NaB.