Fig. 3.

Identification of DNA/protein interaction involving butyrate regulation at the human NHE8 gene promoter by gel mobility shift assays (GMSAs). Oligo DNA was end-labeled with γ³²P-ATP and used as a probe in GMSAs. Nuclear protein was isolated from CT or NaB. GMSAs were performed as indicated in the method section. GC-box consensus oligo (SP Cons.) was used to compete Sp protein family binding. Sp1 and Sp3 antibodies (αSp1 and αSp3) were used for supershift experiments in the presence of labeled probe. ss, Supershift complex.