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. 2015 Jul 8;309(6):G500–G505. doi: 10.1152/ajpgi.00194.2015

Fig. 4.

Fig. 4.

Effect of butyrate on Sp3 protein in human intestinal epithelial cells. Caco-2 cells were cultured in standard medium or butyrate-containing medium (10 mM, 16 h). Nuclear protein was isolated and used for Western blot detection. Sp3 protein abundance is calculated as a ratio of Sp3 intensity over TBP protein intensity. The degree of Sp3 protein expression is shown as the ratio of Sp3 protein abundance in butyrate-treated cells over Sp3 protein abundance in control cells. Results are means ± SE from 4 separate experiments. *P < 0.05 for CT vs. NaB. Top: a representative Western image; bottom: summary results of Sp3 protein abundance in the nuclei.