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. 2015 Jul 8;309(6):G500–G505. doi: 10.1152/ajpgi.00194.2015

Fig. 5.

Fig. 5.

Effect of trichostatin (TSA) on human NHE8 gene promoter activity and Sp3 protein expression. A: effect of TSA on human NHE8 gene promoter is shown. Cells were cotransfected with pRL-CMV and pGL3 basic (pGL3b) or human NHE8 promoter construct pGL3b/-32. TSA (1 μM) was applied 16 h before measuring promoter activities. The degree of stimulation is shown as the ratio of relative luciferase activity in TSA-treated cells over relative luciferase activity in control cells. Results are means ± SE from 4 separate experiments. *P < 0.05 for CT vs. TSA treatment (TSA). B: effect of TSA on Sp3 protein expression. Caco-2 cells were cultured in standard medium or TSA-containing medium (1 μM, 16 h). Nuclear protein was isolated and used for Western blot detection. Sp3 protein abundance is calculated as a ratio of Sp3 intensity over TBP protein intensity. The degree of Sp3 protein expression is shown as the ratio of Sp3 protein abundance in TSA-treated cells over Sp3 protein abundance in control cells. Results are means ± SE from 3 separate experiments. *P < 0.05 for CT vs. TSA. B, top: representative Western image; B, bottom: summary results of Sp3 protein expression in nuclear extracts.