Fig. 3.
SCN5A is expressed in human colonic smooth muscle. A: reverse transcription PCR (RT-PCR) using primers specific for SCN5A was carried out on RNA extracted from the human colonic smooth muscle layer. Identity of the band was confirmed by sequencing. From left to right, human colon smooth muscle, negative control lacking reverse transcriptase, blank lane, DNA ladder. GAPDH was used as a positive control for extraction. Black arrowhead points to 500 bp. B: protein immunoblots from human heart and human colon circular smooth muscle from three different samples demonstrate expression of Nav1.5.