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. 2015 Jul 30;309(6):G491–G499. doi: 10.1152/ajpgi.00140.2015

Table 1.

Primers used for RT-PCR

Primers
Gene GenBank Accession Number Sense Anti-Sense Product Size, bp
Twist1 (mouse) NM_011658 5′ CGACGACAGCCTGAGCAACA 3′ 5′ TGCAGCTCCTCGTACGACTG 3′ 293
CCN2 (mouse) NM_010217 5′ CACTCTGCCAGTGGAGTTCA 3′ 5′ AAGATGTCATTGTCCCCAGG 3′ 111
miR-214 (mouse) NR_029796 5′ ACAGCAGGCACAGACAGGCA 3′ Universal anti-sense 20
Collagen α1(I) (mouse) NM_007742 5′ GCCCGAACCCCAAGGAAAAGAAGC 3′ 5′ CTGGGAGGCCTCGGTGGACATTAG 3′ 148
α−SMA (mouse) NM_007392 5′GGCTCTGGGCTCTGTAAGG3′ 52CTCTTGCTCTGGGCTTCATC3′ 148
GAPDH (mouse) NM_002046 5′ TGCACCACCAACTGCTTAGC 3′ 5′ GGCATGGACTGTGGTCATGAG 3′ 87

CCN2, connective tissue growth factor; SMA, smooth muscle actin.