Table 1.
Primers |
||||
---|---|---|---|---|
Gene | GenBank Accession Number | Sense | Anti-Sense | Product Size, bp |
Twist1 (mouse) | NM_011658 | 5′ CGACGACAGCCTGAGCAACA 3′ | 5′ TGCAGCTCCTCGTACGACTG 3′ | 293 |
CCN2 (mouse) | NM_010217 | 5′ CACTCTGCCAGTGGAGTTCA 3′ | 5′ AAGATGTCATTGTCCCCAGG 3′ | 111 |
miR-214 (mouse) | NR_029796 | 5′ ACAGCAGGCACAGACAGGCA 3′ | Universal anti-sense | 20 |
Collagen α1(I) (mouse) | NM_007742 | 5′ GCCCGAACCCCAAGGAAAAGAAGC 3′ | 5′ CTGGGAGGCCTCGGTGGACATTAG 3′ | 148 |
α−SMA (mouse) | NM_007392 | 5′GGCTCTGGGCTCTGTAAGG3′ | 52CTCTTGCTCTGGGCTTCATC3′ | 148 |
GAPDH (mouse) | NM_002046 | 5′ TGCACCACCAACTGCTTAGC 3′ | 5′ GGCATGGACTGTGGTCATGAG 3′ | 87 |
CCN2, connective tissue growth factor; SMA, smooth muscle actin.