Table 1.
Nucleotide sequence and E-box elements identified in silico using MatInspector.
| Identified E-box | Species | Sequence |
|---|---|---|
| AR E-box -1135 | Human | 5′-atgccaCGAGgcc-3′ |
| Mouse | 5′-gt gtcaggaattc-3′ | |
|
| ||
| LRH-1 E-box -53 | Human | 3′-tcatcaCATGact-5′ |
| Mouse | 3′-tggtcacatgacc-5′ | |
|
| ||
| LRH-1 E-box -1300 | Human | 5′-tggccaGGTGcgg-3′ |
| Mouse | 5′-ttgccttcagtgc-3′ | |
|
| ||
| SHP E-box -177 | Human | 5′-gtgccaCGTGggg-3′ |
| Mouse | 5′-aggccacgtggagc-3′ | |
|
| ||
| SHP E-box -3702 | Human | 3′-ggaTCACttgagg-5′ |
| Mouse | 3′-agatctctcagct-3′ | |
E-box elements were named based on distance to transcriptional start, as shown in Figure 3. Core human sequence elements, as identified by MatInspector (see Section 2), are shown in capitals.
Nucleotides conserved, in human and mouse, are shown underlined in the mouse sequence; sequence alignment was done using Clustalw2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/), under default settings.