Table 2.

Preferential interaction potential for trehalose

	$\frac{10000\ast {\mu }_{23}}{\text{RT}}/{\text{ASA}}_{\text{i}}$ (m−1 Å−2)
Aliphatic C	22.4 ± 0.8
Aromatic C	5.9 ± 1.5a, −8.9 ± 1.6b
Hydroxyl O	−0.8 ± 0.7
Amide O	−19.6 ± 5.4
Amide N	−4.7 ± 2.2
Carboxylate O	−28.2 ± 2.1
Cationic N	12.9 ± 2.1
Phosphate O	−59.2 ± 5.1
	${\mu }_{23}\text{/RT}$ (m−1)
Na+	0.09 ± 0.03
Cl−	0.00 ± 0.03

Except for the second value of Aromatic C, the error is given by multiple linear regression analysis of ${\mu }_{23}\text{/RT}$ as a linear combination of the ASA composition of model compounds, using the uncertainty of ${\mu }_{23}\text{/RT}$ as reported in Table 1.

^aFor interaction of trehalose with benzene C of Phe.

^bFor interaction of trehalose with aromatic ring atoms in imidazole (N and C) or phenol; calculated as the average value for His and 4BA.