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. 2015 Jul 10;309(6):L584–L592. doi: 10.1152/ajplung.00211.2014

Fig. 3.

Fig. 3.

Microvessel permeability in LPS-instilled lungs. Permeability of single microvessels in isolated blood-perfused rat lungs was determined by infusing FITC-tagged dextran 20 kDa (FDx20) into the microvessels, followed by a 10-min Ringer's washoff, as detailed in materials and methods. Fluorescence images were captured during the FDx20 infusion and subsequent washoff. A: image shows maximal FDx20 fluorescence in microvessels during FDx20 infusion in Ringer's instilled (control) lungs. B–D: images show residual FDx20 fluorescence after a Ringer's wash to remove luminal FDx20 in control lungs (B) and LPS-instilled lungs that were allowed to recover for 24 h (C) and 5 days (D). E: bar graph shows ratio of maximal to residual FDx20 fluorescence (normalized fluorescence) in single lung microvessels of rats allowed to recover from LPS challenge for durations shown. §P < 0.05 compared with all other treatment groups. n, no. of vessels analyzed. (3 lungs/treatment group).