Table 1.
Primers employed
| Gene Name | Slc26A4 | TH | RNA18S5 | Actb |
|---|---|---|---|---|
| Protein | Pendrin | Tyrosine hydroxylase | None | β-Actin |
| Species | rat | Rat | Human | Rat |
| Accession no. | NM_019214 | NM_012740 | NR_003286 | NM_031144 |
| Synonyms | Pds | The | RN18S1 | Actx |
| Forward Primer | 911+ CAGTCCCGATTCCTATAG | 1281+ CAAGATCAAACCTACCAG | 1335+ CGGCTTAATTTGACTCAACAC | 1032+ TACTGCCCTGGCTCCTAGC |
| Reverse Primer | 988− AATTTGCTTCCAAGTTGG | 1366− ATACGAGAGGCATAGTTC | 1401− ATCAATCTGTCAATCCTGTCC | 1113− AGAGCCACCAATCCACACA |
| Probe | 940+ FAM-ACAATTATCGCCACCGCCA-BHQ1 | 1338+ FAM-CCTTGGCGTCATTGAAGCTCTC-BHQ1 | 1359+ FAM-AAACCTCACCCGGCCCG-BHQ1 | 1060+ FAM-ATCAAGATCATTGCTCCTCCTGAGCGC-BHQ1 |
| Amplicon Length, bases | 78 | 86 | 67 | 82 |
| PCR Efficiency, % | 100 | 97 | 99 | 98 |
| LOQ, copies | 20 | 20 | 2000 | 20 |
| Exon Boundary | 7/8 | 13/14 | N/A | 5/6 |
| Splice Variants | All detected | None | None | All detected |
| m-Fold | None | None | None | None |
| BLAST | Specific | Specific | Specific | Specific* |
LOQ, limit of quantification.
*
Might detect γ-actin.