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. 2015 Jul 7;109(1):57–65. doi: 10.1016/j.bpj.2015.06.003

Figure 5.

Figure 5

Detachment by FA disconnection is ubiquitous in mammalian cells in media containing cations at 37°C. (Left) Fluorescence images of Wi38 and 3T3 fibroblasts on top and middle, respectively, were stained for DNA (blue), actin (red), and paxillin (green). On the bottom, 3T3 fibroblasts are shown in brightfield (gray) with the FAK (green) image overlaid. Images are shown without shear (left) and after shear-induced cell detachment (right). Arrows indicate shear direction. Arrowheads denote focal adhesion left on the coverslip after detachment. To see this figure in color, go online.