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. 2015 Jul 7;109(1):7–17. doi: 10.1016/j.bpj.2015.05.035

Figure 5.

Figure 5

FP flickering gives rise to the plateaus we see for larger D values. We used FCS data acquired for freely diffusing Alexa568, which is well behaved, as a control for our experimental data to determine if it would show a superdiffusive plateau in our analysis: results were negative (a). We then performed a similar analysis for mCherry and mRuby2 freely diffusing while flickering and extracted the resulting p(D) from G(τ) using our method: a superdiffusive plateau was reproduced; we show here the data for mRuby2 (b) 56. We conclude that the superdiffusive plateaus in the p(D) s extracted from our data sets are consistent with flickering of the FP. Furthermore, we conclude the remaining features at lower D values in p(D) that may arise from binding of TFs to DNA are well separated from the flickering plateau if it arises in the data. The small peak at low values of D for Alexa568 (a) is due to FP aggregation. To see this figure in color, go online.