TABLE 4.

Low-level variants of BKV HLA-A2-restricted peptide sequences^a detected in clinical samples of pediatric kidney and hematopoietic cell transplant recipients

Antigen position	Peptide sequence	Mutation	No. of patients	Variant frequency (%)b
VP1 antigen
BKV VP1 p62	NLRGFSLKL	F66L	1	1.87
BKV VP1 p84	KMLPCYSTA	L86P	1	1.87
BKV VP1 p108	LLMWEAVTV	E112G	1	2.38
BKV VP1 p128	NLHAGSQKV	L129F	1	1.72
		Q134P	1	4.49
		K135Q	1	5.34
BKV VP1 p245	LLDEQGVGPL	L254F	1	1.67
BKV VP1 p259	SLYVSAADI	L260P	1	3.62
Large T antigen
BKV T p157	TLACFAVYT	V163A	1	3.03
BKV T p176	KLMEKYSVT	M178I	3	2.26; 2.30; 3.53
		E179G	2	2.25; 2.27
		K180N	5	1.92; 2.34; 2.34; 2.49; 3.27
BKV T p199	FLTPHRHRV	H203R	1	1.96
BKV T p216	KLCTFSFLI	K216R	1	1.88
		C218R	1	1.79
		T219A	1	1.93
		T219N	1	2.39
		F220L	1	4.03
		S221R	1	3.31
		S221C	1	3.3
		S221N	1	3.18
		I224F	2	2.00; 2.37
BKV T p313	PYHFKYHEKHFANAT	K321E	1	2.04
		T327N	1	1.97
BKV T p362	MLTERFNHIL	T364A	1	1.94
		F367S	2	1.98; 3.22
BKV T p395	WLHCLLPKM	H397R	1	2.07
BKV T p398	CLLPKMDSV	S405F	1	4.02
BKV T p406	VIFDFLHCI	D409E	3	1.44; 1.65; 2.21
		D409G	2	1.56; 2.61
		D409Y	2	1.55; 2.74
BKV T p410	FLHCIVFNV	L411S	1	4.03
BKV T p558	SLQNSEFLL	N561T	1	2.1
		S562P	1	3.36
BKV T p570	ILQSGMTLL	I570F	2	2.05; 2.19
Small T antigen
BKV T p4	VLNREESME	R7G	1	1.8
BKV T p10	SMELMDLLGL	E12V	1	9.28
BKV T p125	FLRKEPLVWI	R127G	2	1.51; 1.86
		K128R	1	1.85

^aLow-level variants of BKV HLA-B0702- and HLA-B08-restricted peptide sequences were not identified.

^bVariants that appeared in multiple specimens for a patient were averaged. Frequencies for each patient are listed.