TABLE 2.
Characteristics of the 149 M. pneumoniae isolates analyzed using the SNP typing method
| Straina | Year of isolation | Macrolide resistanceb | Geographical origin | SNP type | MLVA-4 profilec | MLVA-5 type | P1 gene type | Reference or source |
|---|---|---|---|---|---|---|---|---|
| M129 | Unknown | USA | SNP5 | 4572 | P | 1 | ATCC 29342 | |
| B1145d | 1994 | Brest/France | SNP9 | 4572 | P | 1 | 15 | |
| B3996d | 2005 | R | Bordeaux/France | SNP2 | 3572 | N | 1 | 15 |
| B4560d | 2007 | Bayonne/France | SNP1 | 4572 | E | 1 | 15 | |
| M2285d | 1995 | Madrid/Spain | SNP3 | 4572 | E | 1 | 15 | |
| J382d | 2000-2003 | R | Japan | SNP4 | 4572 | P | 1 | 15 |
| F59d | Unknown | Germany | SNP6 | 3562 | V | 2a | 15 | |
| B3896d | 2005 | Bordeaux/France | SNP7 | 3562 | S | 2-related variant | 48 | |
| FHd | 1954 | Boston, MA/USA | SNP8 | 3662 | T | 2 | ATCC 15531 | |
| B5767 | 2011 | Bordeaux/France | SNP9 | 4572 | J | 1 | This study | |
| B5596 | 2009 | Bordeaux/France | SNP1 | 4572 | J | 1 | This study | |
| B5612 | 2009 | Bordeaux/France | SNP2 | 4572 | E | 1 | This study | |
| B5029 | 2009 | Bordeaux/France | SNP8 | 3562 | M | 2 | This study | |
| B4997 | 2008 | Bordeaux/France | SNP9 | 4572 | X | 1 | This study | |
| J376 | 2000-2003 | R | Japan | SNP8 | 3662 | C | 2 | 15 |
| J380 | 2000-2003 | R | Japan | SNP4 | 4572 | J | 1 | 15 |
| J377 | 2000-2003 | R | Japan | SNP8 | 3662 | C | 2 | 15 |
| B3737 | 2004 | Bordeaux/France | SNP1 | 4572 | X | 1 | 15 | |
| B4692 | 2007 | Bordeaux/France | SNP9 | 4472 | 31e | 1 | 15 | |
| B4879 | 2008 | Bordeaux/France | SNP9 | 4472 | 31e | 1 | This study | |
| B5475 | 2009 | Bordeaux/France | SNP6 | 3562 | M | 2 | This study | |
| B4709 | 2007 | Bordeaux/France | SNP9 | 4572 | J | 1 | 15 | |
| B3766 | 2004 | Bordeaux/France | SNP2 | 4572 | U | 1 | 15 | |
| L3 | 1995 | Lyon/France | SNP9 | 4572 | X | 1 | 15 | |
| L19 | 1997 | Lyon/France | SNP8 | 2662 | L | 2 | 15 | |
| FG9 | 1997 | St-Etienne/France | SNP1 | 4572 | P | 1 | 15 | |
| FG8 | 1997 | St-Etienne/France | SNP3 | 4572 | E | 1 | 15 | |
| J22 | 1993 | Germany | SNP1 | 4572 | Z | 1 | 15 | |
| B4578 | 2007 | Bordeaux/France | SNP9 | 4572 | E | 1 | 15 | |
| J17 | 1992 | Germany | SNP3 | 4572 | P | 1 | 15 | |
| J11 | 1992 | Germany | SNP1 | 4572 | X | 1 | 15 | |
| B4112 | 2006 | Bordeaux/France | SNP6 | 3562 | M | 2 | 15 | |
| B2892 | 1999 | Bordeaux/France | SNP8 | 3662 | O | 2 | 15 | |
| B936 | 1994 | Bordeaux/France | SNP1 | 4572 | J | 1 | 15 | |
| B4602 | 2007 | Bayonne/France | SNP1 | 4572 | J | 1 | 15 | |
| Pn22 | 1992 | Bordeaux/France | SNP1 | 3572 | R | 1 | 15 | |
| L59 | 2000 | Lyon/France | SNP8 | 3572 | N | 2 | 15 | |
| B3885 | 2005 | Bordeaux/France | SNP1 | 4572 | P | 1 | 15 | |
| B4223 | 2006 | Bordeaux/France | SNP1 | 4572 | J | 1 | 15 | |
| B3119 | 2001 | Bordeaux/France | SNP6 | 3562 | V | 2 | 15 | |
| B3163 | 2001 | Bordeaux/France | SNP8 | 3662 | C | 2 | 15 | |
| B3448 | 2003 | Bordeaux/France | SNP8 | 3662 | C | 2 | 15 | |
| Pn15 | 1992 | Bordeaux/France | SNP9 | 4572 | J | 1 | 15 | |
| Pn21 | 1992 | Bordeaux/France | SNP9 | 4572 | U | 1 | 15 | |
| N35 | 1986 | Nantes/France | SNP6 | 3562 | V | 2 | 15 | |
| FG10 | 1997 | St-Etienne/France | SNP9 | 4572 | U | 1 | 15 | |
| 79692 | 2000 | Germany | SNP6 | 3562 | M | 2a | 15 | |
| Sta | 1995 | Germany | SNP6 | 3562 | M | 2a | 15 | |
| C83 | 2006 | Caen/France | SNP8 | 3662 | T | 2 | 15 | |
| C71 | 2005 | Caen/France | SNP1 | 4572 | Z | 1 | 15 | |
| B3869 | 2005 | Bordeaux/France | SNP2 | 4572 | U | 1 | 15 | |
| B4100 | 2006 | Bordeaux/France | SNP9 | 4572 | E | 1 | 15 | |
| B2829 | 1999 | Bordeaux/France | SNP1 | 4572 | U | 1 | 15 | |
| AV3 | 1992 | Anvers/Belgium | SNP9 | 4572 | P | 1 | 15 | |
| FG12 | 1997 | St-Etienne/France | SNP9 | 4572 | J | 1 | 15 | |
| Pn19 | 1992 | Bordeaux/France | SNP3 | 4572 | E | 1 | 15 | |
| J16 | 1992 | Germany | SNP4 | 4572 | P | 1 | 15 | |
| L37 | 1997 | Lyon/France | SNP9 | 4572 | U | 1 | 15 | |
| B3722 | 2004 | Bordeaux/France | SNP2 | 4572 | U | 1 | 15 | |
| B6254 | 2012 | Bordeaux/France | SNP1 | 4572 | P | 1 | This study | |
| B6199 | 2012 | Bordeaux/France | SNP1 | 4572 | U | 1 | This study | |
| B6205 | 2012 | Bordeaux/France | SNP5 | 4672 | 37e | 1 | This study | |
| B6303 | 2012 | Bordeaux/France | SNP2 | 4572 | P | 1 | This study | |
| B6203 | 2012 | Bordeaux/France | SNP1 | 4572 | U | 1 | This study | |
| C6 | 2011 | Caen/France | SNP1 | 4572 | P | 1 | This study | |
| C43 | 2011 | Caen/France | SNP8 | 3662 | T | 2b | This study | |
| C2 | 2011 | Caen/France | SNP1 | 4572 | P | 1 | This study | |
| C17 | 2011 | Caen/France | SNP1 | 4572 | P | 1 | This study | |
| C40 | 2011 | Caen/France | SNP9 | 4572 | J | 1 | This study | |
| B6094 | 2012 | Bordeaux/France | SNP9 | 4572 | J | 1 | This study | |
| B6102 | 2012 | Bordeaux/France | SNP9 | 4572 | E | 1 | This study | |
| B6148 | 2012 | Bordeaux/France | SNP9 | 4572 | E | 1 | This study | |
| B6074 | 2012 | Bordeaux/France | SNP9 | 4572 | E | 1 | This study | |
| B6085 | 2012 | Bordeaux/France | SNP1 | 3572 | 39e | 1 | This study | |
| B6128 | 2012 | Bordeaux/France | SNP9 | 4572 | U | 1 | This study | |
| B5973 | 2011 | Bordeaux/France | SNP6 | 3562 | G | 2b | This study | |
| B5927 | 2011 | Bordeaux/France | SNP9 | 4572 | P | 1 | This study | |
| B6009 | 2011 | Bordeaux/France | SNP6 | 3562 | V | 2a | This study | |
| B6052 | 2011 | Bordeaux/France | SNP9 | 4572 | P | 1 | This study | |
| Mpnlim | 2011 | R | Limoges/France | SNP1 | 3672 | I | 1 | This study |
| B6318 | 2012 | Bordeaux/France | SNP8 | 3662 | T | 2b | This study | |
| B5674 | 2010 | Bordeaux/France | SNP5 | 4572 | P | 1 | This study | |
| B5959 | 2011 | Bordeaux/France | SNP9 | 4672 | 29e | 1 | This study | |
| B3568 | 2003 | Bordeaux/France | SNP1 | 4572 | A | 1 | 15 | |
| B3487 | 2003 | Bordeaux/France | SNP1 | 4572 | A | 1 | 15 | |
| B718 | 1993 | Bordeaux/France | SNP3 | 4562 | D | 1 | 15 | |
| L15 | 1997 | Lyon/France | SNP8 | 3662 | W | 2 | 15 | |
| B2085 | 1996 | Bordeaux/France | SNP8 | 3662 | W | 2 | 15 | |
| L10 | 1996 | Lyon/France | SNP1 | 4571 | Q | 1 | 15 | |
| B2918 | 2000 | Bordeaux/France | SNP1 | 4571 | Q | 1 | 15 | |
| C76 | 2006 | Caen/France | SNP8 | 3662 | W | 2 | 15 | |
| J173 | Unknown | R | Japan | SNP1 | 4572 | A | 1 | 15 |
| J186 | Unknown | R | Japan | SNP1 | 4572 | A | 1 | 15 |
| J381 | 2000-2003 | R | Japan | SNP5 | 4562 | K | 1 | 15 |
| B3098 | 2000 | Argenteuil/France | SNP9 | 4582 | F | 1 | 15 | |
| B4466 | 2007 | Bordeaux/France | SNP6 | 3562 | S | 2 | 15 | |
| B3836 | 2004 | Bordeaux/France | SNP8 | 3662 | C | 2 | 15 | |
| B4079 | 2005 | Bordeaux/France | SNP8 | 3662 | W | 2 | This study | |
| B6048 | 2012 | Bordeaux/France | SNP9 | 4572 | U | 1 | This study | |
| B6056 | 2012 | Bordeaux/France | SNP5 | 4572 | X | 1 | This study | |
| B6021 | 2011 | Bordeaux/France | SNP1 | 4572 | U | 1 | This study | |
| B6096 | 2012 | Bordeaux/France | SNP1 | 4572 | P | 1 | This study | |
| B6028 | 2012 | Bordeaux/France | SNP9 | 4572 | J | 1 | This study | |
| B5938 | 2011 | Bordeaux/France | SNP6 | 3562 | G | 2c | This study | |
| B5904 | 2011 | Bordeaux/France | SNP9 | 4572 | J | 1 | This study | |
| B5847 | 2011 | Bordeaux/France | SNP1 | 4572 | X | 1 | This study | |
| B5837 | 2011 | Bordeaux/France | SNP1 | 4572 | X | 1 | This study | |
| B5817 | 2011 | Bordeaux/France | SNP1 | 4572 | U | 1 | This study | |
| B6312 | 2012 | Bordeaux/France | SNP6 | 3562 | G | 2a | This study | |
| B5336 | 2009 | Bordeaux/France | SNP9 | 4672 | 29e | 1 | This study | |
| B5719 | 2010 | Bordeaux/France | SNP9 | 4572 | P | 1 | This study | |
| B4747 | 2008 | Bordeaux/France | SNP6 | 3562 | B | 2 | This study | |
| B5563 | 2010 | Bordeaux/France | SNP9 | 4572 | E | 1 | This study | |
| B4972 | 2008 | R | Bordeaux/France | SNP8 | 4572 | Z | 2 | This study |
| B5776 | 2011 | Bordeaux/France | SNP9 | 4572 | J | 1 | This study | |
| B5954 | 2011 | R | Bordeaux/France | SNP2 | 4572 | P | 1 | This study |
| B6329 | 2012 | R | Bordeaux/France | SNP8 | 3662 | C | 2 | This study |
| C95 | 2007 | Caen/France | SNP8 | 3662 | H | 2 | 15 | |
| C98 | 2007 | Caen/France | SNP8 | 3662 | H | 2 | 15 | |
| C89 | 2006 | Caen/France | SNP8 | 3662 | H | 2 | 15 | |
| L8 | 1996 | Lyon/France | SNP8 | 3562 | B | 2 | 15 | |
| L62 | 2000 | Lyon/France | SNP8 | 3562 | M | 2 | 15 | |
| B4561 | 2007 | Bayonne/France | SNP6 | 3562 | S | 2 | 15 | |
| L18 | 1997 | Lyon/France | SNP8 | 3662 | C | 2 | 15 | |
| AV1 | 1992 | Anvers/Belgium | SNP8 | 3662 | C | 2 | 15 | |
| AV2 | 1992 | Anvers/Belgium | SNP3 | 4572 | E | 1 | 15 | |
| AV4 | 1992 | Anvers/Belgium | SNP3 | 4572 | E | 1 | 15 | |
| AV8 | 1993 | Anvers/Belgium | SNP3 | 4572 | E | 1 | 15 | |
| AV9 | 1993 | Anvers/Belgium | SNP3 | 4572 | E | 1 | 15 | |
| AV6 | 1993 | Anvers/Belgium | SNP1 | 4572 | J | 1 | 15 | |
| AV7 | 1993 | Anvers/Belgium | SNP9 | 4572 | J | 1 | 15 | |
| A131 | 2006 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A167 | 2006 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A168 | 2006 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A195 | 2006 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A251 | 2007 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A340 | 2008 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| A355 | 2008 | Tunisia | SNP5 | 4572 | P | 1 | 15 | |
| M5 | 1962 | Denmark | SNP5 | 4572 | P | 1 | 15 | |
| M40 | 1963 | Denmark | SNP5 | 4572 | P | 1 | 15 | |
| M38 | 1963 | Denmark | SNP5 | 4572 | Z | 1 | 15 | |
| M62 | 1964 | Denmark | SNP5 | 4572 | X | 1 | 15 | |
| M547 | 1967 | Denmark | SNP8 | 3662 | T | 2 | 15 | |
| M1121 | 1977 | Denmark | SNP4 | 4572 | J | 1 | 15 | |
| M1873 | 1987 | Denmark | SNP8 | 3662 | C | 2 | 15 | |
| M2018 | 1988 | R | Denmark | SNP4 | 4572 | P | 1 | 15 |
| M2155 | 1990 | Denmark | SNP9 | 4572 | P | 1 | 15 | |
| M4350 | 1991 | Denmark | SNP8 | 3562 | V | 2 | 15 | |
| 4817 | 1993 | Denmark | SNP1 | 4572 | J | 1a | 15 |
a
All strains were isolated from respiratory tract specimens (sputum samples, bronchoalveolar lavage [BAL] fluid samples, throat swabs, nasopharyngeal aspirates, and tracheal aspirates) except for strains B1145, M2285, and B3163 isolated from cerebrospinal fluid, pericardial fluid, and sternal wound specimens, respectively.
b
Only macrolide-resistant isolates are indicated by R. All other isolates were susceptible to macrolides.
c
Order of VNTRs: Mpn13, Mpn14, Mpn15, Mpn16.
d
Isolates submitted to whole-genome sequencing.
e
MLVA-5 types are named according to reference 15 and MLVA profiles of MLVA-5 types 29, 31, 37, and 39 are 24672, 84472, 54672, and 63572, respectively.