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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Dev Biol. 2015 Jul 8;405(2):225–236. doi: 10.1016/j.ydbio.2015.07.006

Figure 2.

Figure 2

BDNF expression is reduced in circumvallate taste buds during late postnatal development. β-Gal activity was detected in the circumvallate (CV) taste buds of BdnfLacZ/+ mice from birth to adulthood (A-E), but not in wild-type mice (F). β-Gal staining was observed in the entire circumvallate trench wall from P0 to P10 (A-C). However, the staining was restricted within taste buds by P20 (D) and P60 (E), and some taste bud cells were negative for β-Gal (arrows). (G) RT-PCR confirmed a decrease in BDNF expression in the circumvallate papilla epithelium across postnatal ages. The expression of BDNF in circumvallate taste buds was not different between birth and P10, but BDNF expression levels gradually decreased from P0 to P20 (p = 0.02). There was no difference in BDNF expression between P20 and P60. The scale bar in F applies to panels A-F. The error bars in G represent the standard error, and the single asterisk represents p ≤ 0.05.