Table 1. The antibodies used in the study.

Antibody	Host	Concentration	Company	Cat. #
5-HT	Rabbit	1:500	ImmunoStar	20080
β-Gal	Rabbit	1:1000	MP Biomedicals	55976
Car4	Goat	1:500	R&D Systems	AF2414
Keratin-8	Rat	1:50	Developmental Studies Hybridoma Bank	Troma-1
NTPDase2	Rabbit	1:500	CHUL	mN2-35L; mN2-36L
P2X3	Rabbit	1:500	Millipore	AB5895
PCLβ2	Rabbit	1:500	Santa Cruz	sc-206
SNAP25	Rabbit	1:500	Millipore	AB1762

BDNF expression in taste buds decreases between postnatal days 5 and 10 BDNF is reduced specifically from progenitor cells and not taste receptor cells Innervation density in the taste bud is reduced between postnatal days 5 and 10 BDNF overexpression blocks the refinement of innervation